taq pcr core kit with q-solution (Qiagen)

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Qiagen taq pcr core kit with q-solution
Taq Pcr Core Kit With Q Solution, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/core+solution/quantitect+reverse+transcription+kit/pm40490511-317-18-19
Average 90 stars, based on 1 article reviews

taq pcr core kit with q-solution - by Bioz Stars, 2026-10

90/100 stars

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Centrifugation:

Article Title: Characterization and quantification of the fungal microbiome in serial samples from individuals with cystic fibrosis
Article Snippet: To remove RNA, RNase (1.5 μl) (QIAGEN Inc.) was added and the samples were incubated for 30 min at 37°C. .. Lysates were chilled on ice for 1 min, 200 μl of Protein Precipitation Solution (Qiagen Puregene Core Kit B, QIAGEN Inc.) was added, and the solutions were mixed vigorously for 20 s. Cell debris was sedimented by centrifugation at 13,000 rpm for 3 min, and the supernatant was transferred to a new 1.5-ml tube prior to addition of 600 μl of 100% isopropanol. ..

Polymerase Chain Reaction:

Article Title: PMS1 as a target for splice modulation to prevent somatic CAG repeat expansion in Huntington’s disease
Article Snippet: .. We used the Taq PCR Core Kit with Q solution (Qiagen, 201225) with 5 μL of the isolated genomic DNA following PCR conditions: initial denaturation 95 °C (5 min), 30 cycles of 95 °C (30 s), 65 °C (30 s), 72 °C (1 min 30 s), final extension 72 °C (10 min). ..

Article Title: Development and validation of a new mpox virus sequencing and bioinformatic analysis pipeline
Article Snippet: .. MPXV DNA was subjected to five PCRs, amplification using primer pool A, B, C, D or E. Each PCR reaction of 25 μL included 6 μL of master mix solution (AllTaq PCR Core Kit®, Qiagen, USA), 1 μL of primer pool A, B, C, D or E, 5 μL of MPXV DNA and 13 μL of nuclease free water. .. The following thermal cycling conditions were used on an Eppendorf Mastercycler® X50 (Eppendorf, USA): initial denaturation at 95°C for 2 min, followed by 40 cycles at 95°C for 5 s and 50°C for 15 s, and a final extension of 72°C for 10 minutes.

Article Title: Development and Validation of a New Mpox Virus Sequencing and Bioinformatic Analysis Pipeline.
Article Snippet: .. MPXV DNA was subjected to five PCRs, amplification using primer pool A, B, C, D or E. Each PCR reaction of 25 μL included 6 μL of master mix solution (AllTaq PCR Core Kit®, Qiagen, USA), 1 μL of primer pool A, B, C, D or E, 5 μL of MPXV DNA and 13 μL of nuclease free water. .. The following thermal cycling conditions were used on an Eppendorf Mastercycler® X50 (Eppendorf, USA): initial denaturation at 95°C for 2 min, followed by 40 cycles at 95°C for 5 seconds and 50°C for 15 seconds, and a final extension of 72°C for 10 minutes.

Isolation:

Amplification:

Incubation:

Article Title: Mammalian Nudix proteins cleave nucleotide metabolite caps on RNAs
Article Snippet: .. Nudt16 was incubated with 5 mM FAD at room temperature for 5 h. Crystals were obtained by the sitting-drop vapor diffusion method at 20°C using this mixture, with the reservoir solution of 0.2 M ammonium chloride and 20% (w/v) PEG 3350 (JCSG Core Suite I, Qiagen). ..

Diffusion-based Assay:

DNA Extraction:

Article Title: Utility of Self-Destructing CRISPR/Cas Constructs for Targeted Gene Editing in the Retina.
Article Snippet: .. Genomic DNA extraction from mouse retinas and whole exome sequencing Genomic DNA from two retinas treated with SpCas9 sgRNA/YFP sgRNA6 and two contralateral retinas were extracted using commercial Genomic DNA Purification Kits (QIAGEN, Puregene Core Kit A, Lot no. 8570335; RNase A Solution, Catalog no. 158922; Puregene Proteinase K, Catalog no. 158918) and dissolved in TE buffer. .. Whole exome sequencing and standard bioinformatics analyses were performed by Beijing Genomics Institute (BGI).

Sequencing:

DNA Purification:

other:

Article Title: Splice modulators target PMS1 to reduce somatic expansion of the Huntington’s disease-associated CAG repeat
Article Snippet: hTERT RPE-1 (CRL-4000 – ATCC, female origin) were transfected with lipofectamine 3000 (Invitrogen, L3000001) following the manufacturer’s protocol with AAVS1 targeting vector and predesigned transcription activator-like effector nucleases (hAAVS1 TALEN Left and Right were gifts from Su-Chun Zhang, Addgene plasmid # 52341 & 52342; http://n2t.net/addgene:52341 ; http://n2t.net/addgene:52342 ; RRID:Addgene_52341; RRID:Addgene_52342).

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